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Trồng trọt

Hoàng Hoa Long, Phạm Xuân Hội(1)

Phân lập và đặc trưng của chủng vi khuẩn nội sinh kích thích sinh trưởng thực vật từ cây cà dại

Isolation and characterization of plant growth promoting bacterial endophytes from a wild Solanaceae plant species

Nông nghiệp & Phát triển nông thôn

2012

11

7-11

1859-4581

Wild plants harbor a diverse community of bacterial endophytes which can positively affect host plant growth. Changes in plant growth frequently reflect alterations in phytohonnone homoeostasis by plantgrowth-promoting (PGP) rhizobacteria which can decrease ethylene (ET) levels enzymatically by 1aminocyclopropane-1-carboxylate (ACC) deaminase or produce indole-3-acetic acid (IAA). Bacterial endophytes were isolated from roots, stems and leaves of the field-grown Solanum nigrom plant, a native wild Solanaceae plant species; their PGP traits (ACC deaminase activity, IAA production, inorganic phosphate solubilization and seedling colonization) were characterized; and their effects on the seedling vigor were detennined. A majority of isolates that promoted root growth were associated with ACC deaminase activity and IAA production. Colonization of bacterial endophytes inside the root tissues was explored by transforming them with plasmid carrying the gene encoding green fluorescent protein (GFPuv) and observing the inoculated tissues under fluorescence microscope. In short, the research opens a new trend in using native/natural bacterial endophytes with PGP traits from wild plant species to enhance yield of the cultivated crops.

TTKHCNQG, CVv201