Lọc theo danh mục
  • Năm xuất bản
    Xem thêm
  • Lĩnh vực
liên kết website
Lượt truy cập
 Lượt truy cập :  23,904,248
  • Công bố khoa học và công nghệ Việt Nam

62.01

Công nghệ sinh học công nghiệp

Dương Tấn Nhựt, Nguyễn Thị Thu Sương, Phan Quốc Tâm, Vũ Thị Hiền, Nguyễn Bá Nam(1), Lê Kim Cương, Trịnh Thị Hương, Hoàng Xuân Chiến, Hoàng Văn Cương, Phan Ngọc Viên

ảnh hưởng của TDZ và 2,4-D lên khả năng phát sinh phôi vô tính trong nuôi cấy mô lá của giống dâu tây "Mỹ Hương" (Fragaria SPP.)

Effect of TDZ and 2,4-D on direct somatic embryogenesis via leaf tissue culture of strawberry cultivar "My huong" (Faragaria spp.)

Công nghệ Sinh học

2012

2

289-296

1811-4989

Strawberry is one of the most important fruit plant due to high nutritional value (particular in relatively high content vitamin C). Regeneration of strawberry through organogenesis has been studied, but its shoot multiplication was low; beside shoot has abnormalities such as vitrification and curliness on leaf. In order to overcome the weaknesses, in the present study, the authors reported a best regeneration protocol of strawberry through somatic embryogenesis. The leaf explants of strawberry were initially cultured on MS medium containing 1.0 mg/l TDZ and 2,4-D with different concentrations (0.1, 0.2 and 0.3 mg/l); particularly in the experiment contains 1.0 mg/l TDZ and 0.2 mg/l 2,4-D combination which was very effective in inducing somatic embryos (the highest embryos number is 44.83 embryos). Somatic embryos were produced directly from the cut of leaf without intermediary callus phase after two months of culture. Regenerated plants transferred to soil,. showed that normal growth and morphology. This direct somatic embryogenesis from leaf in strawberry is a strong evidence of cell totipotency. The rapid somatic embryo induction protocol would be a useful tool for the propagation and regeneration.

TTKHCNQG, CVv262