Schizochytrium mangrovei PQ6 containsmany important polyunsaturated fatty acids,such as docosahexaenoic acid (DHA, C226-3), eicosahexaenoic acid (EPA, C20 5-3) and docosapentaenoic acid (DPA, C225-6). These fatty acidsare essential forsurvival and growth of many marinefish larvae. In this paper, fresh biomass of S. mangrovei PQ6 was used for culturing rotiferBrachionus plicatilis. In the first formula (L1), rotiferswere fed on baker’s yeast; in second formula(L2), rotifers werefed on mixed two microalgae, Nannochloropsis oculata and Chaetoceros gracilis; in the third formula (L3), rotifers werefed on S. mangroveiPQ6 biomass. Theresults indicated that S. mangrovei PQ6biomass can replace baker’s yeast and autotrophic microalgae in feeding rotifers. Total lipid, total fatty acid and polyunsaturated fatty acid contents of rotifers fed on formula3 (S. mangrovei PQ6) were higher than those within others. Highpolyunsaturated fatty acid (C≥20) in omega-3 and 6 groups; DHA and DPA contents of rotifers fed on S. mangrovei PQ6 were the highest, accounting for 39.81% of total fatty acid;41.95% and 8.24% polyunsaturated fatty acids,corresponding to 20.52; 12.15 and 2.4 mg/g of dried weight, respectively.The survival rate of black sleeper’slarvae was highest whentheywere fed on rotifers grown onL3 formula (51.20 ± 0.89%), followed by L2 formula (48.70 ± 2.67%) and the lowest with L1 formula (43.44 ± 1.54%) (P< 0.05). However, among three formulas, no significant difference was found in the growth of black sleeper’slarvae (P > 0.05). These results suggest that biomass of S. mangroveiPQ6 can replace traditional feedlike autotrophic microalgae or baker’s yeast for biomass culture of the Brachionus plicatilisrotiferfor rearing black sleeper’slarvae to enhance seed quality to meet demands of market.
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