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Dương Tấn Nhựt(2), Hồ Thanh Tâm(1), Nguyễn Thị Thanh Hiền, Lê Kim Cương, Vũ Quốc Luận, Nguyễn Bá Nam, Nguyễn Phúc Huy, Vũ Thị Hiền, Trịnh Thị Hương, Nguyễn Hồng Hoàng, Nguyễn Xuân Tuấn, Nguyễn Thanh Sang, Nguyễn Việt Cường, Đỗ Mạnh Cường, Nguyễn Hoài Châu, Ngô

Khảo sát ảnh hưởng của nano bạc lên sự sinh trưởng và phát triển của cây cúc, dâu tây, đồng tiên nuôi cấy In Vitro

Effects of nanosilver on growth of Chrysanthemum sp., Fragaria sp., and Gerbera sp. cultured in vitro

Công nghệ Sinh học

2014

1

103-111

1811-4989

Nanosilver is a new material, which shows high capabilities in decontamination of microorganism and sterilization. However, effect of nanosilver on plant cell tissue culture has not been researched yet. In this study, nanosilver solution was added into culture media with concentration range from 0 - 20 ml/I for investigation the effect of nanosilver on growth of Chrysanthemum sp., Fragaria sp. and Gerbera sp.cultured in vitro. Different parameters such as plant height, number of leaves and leaves' length, number of roots and roots' length, fresh weight, dry weight and SPAD were determined. Plants stayed healthy in culture better than control treatment. Supplementingwith 10 ml/l nanosilver into culture medium showed the highest growth of Chrysanthemumand Fragaria plantlets while the best growth of Gerberaplantlets was observed in the treatment, which added 5 ml/I nanosilver. However, nanosilver had the negative impact on the growth and development of plants in vitro when increasing concentration in culture medium. Chrysanthemumplantlets of control treatment and 10 ml/l nanosilver treatment were transferred to greenhouse. The Chrysanthemumplantlets derived from 10 ml/I nanosilver treatment also showed the better growth after I and 2 months in ex vitro conditions.

TTKHCNQG, CVv 262