In this study, a total of 31 yeast strains was isolated from 14 different materials (ripe fruits, flowers of fruittree, alcoholic starter, sugarcane bagasse, sawdust, etc.). Eleven isolates could develop well at 42oC and 4 of which was able to grow at temperature up to 45oC (C2, BM2, BM3 va HN4). By testing the fermentative capacity at high temperature and ethanol tolerance. 4 of 11 theses isolates were selected, including HX1. C2. BM2 and BM3, based on their high fennentation peroformance from glucose 2 percent at 40oC and ethanol tolerant level up to 15 percent v/v. These isolates could consume about 16.85 - 16.88 percent v/v glucose (glucose consuming yield was 93.56 - 93.73 percent) in fermentation from saccharified sugar of glutinous rice and about 4.50 -7.00 percent v/v ethanol obtained after 5 fermentation days at 40oC. In fermentation from molasses medium at 20° brix, results showed that these 4 isolates could consume about 13.68 - 13.84 percent v/v glucose (glucose consuming yield was 79.11 - 80.09 percent) and ethanol produced about 3.00 - 3.83 percent v/v. Based on the nucleotide sequence of D1/D2 domain of 265 rDNA analysis and comparison with NCBI database in gene bank (BLAST) that reached 100 percent similarity, the identification results of 4 selected yeast isolated as follow: HX1: Candida tropicalis, BM3: Torulaspora globosa and both C2 and BM2 belonged to Pichia kudriavzevii.
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