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Trồng trọt

Trần Hoàng Dũng(1), Trần Lệ Trúc Hà, Vũ Thị Huyền Trang, Đỗ Thành Trí, Trần Duy Dương

ứng dụng công nghệ ADN để phân loại và nhận diện lan hoàng thảo trầm rừng (dendrobium parishii) và phi điệp (dendrobium anosmum) tại Việt Nam

Application of technology in taxonomy and identification of wild orchids Hoang Thao (Dendrobium parishil) and Phi Diep (Dendrobium anosmum) in Vietnam

Nông nghiệp & Phát triển nông thôn

2012

18

3-9

1859-4581

Several of the orchid species with a powerful fragrance such as Dendrobium parishii and Dendrobium anosmum have attracted the attention of botanists because of their unique biological characters. The identification of species based on morphology is difficult even though they are flowering. In this study, 10 samples of Dendrobium parishii and Dendrobium anosmum were collected and differenced by the sequences of partial rDNA (ribosomal DNA). Nine of them were successfully to amplify and sequences for ITS1, 5.8S and ITS2 (internal transcribed spacer) region in which the lengths of PCR products are 750 to 850 bp. The phylogenetic analysis showed that the ITS sequences help to resolve the position of D. parishii and D. anosmum undoubtedly in the trees. Several samples were revised from Dendrobium parishii to Dendrobium anosmum according to their ITS sequences identify and position in the evolutionary trees.

TTKHCNQG, CVv201