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  • Công bố khoa học và công nghệ Việt Nam

62.33

Công nghệ sinh học công nghiệp

Đỗ Minh Trung(1), Phạm Văn Trân, Nguyễn Bảo Trân, Trần Hải Anh, Toshiko Yoshida, Toshio Nikaido

Nghiên cứu phân lập, nuôi cấy và nhận biết tế bào gốc từ màng ối người

Isolation, culture and characterisation of stem cells from human amniotic membrane

Sinh lý học Việt Nam

2012

1

36-41

1859-2376

Stem cells are defined by their capacity of self-renewal and generate functional differentiated cells that replenish lost cells throughout an organism's lifetime. In recent years human amniotic membrane has been recognised as a possible reserve source of stem cells that may be useful for clincal application in regenerative medicine. The aim of this study was to establish a new cell source that can be used for research and treatment; therefore, this study made isolation, proliferation cultures, and identification of expression for the markers of the stem cells isolated from human amniotic membrane. The results showed that the isolated cells in culture medium had a typical shape, and they expressed positive markers for stem cells and amniotic epithelial cells as Oct-4; SSEA4, Sox2, Vimentin, CK18. Those findings indicated that stem cells from human amniotic membrane were isolated and identified.

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