This study was conducted from Jul to Nov 2019 at the Noi chicken farm in Phong Dien district, Can Tho city to evaluate the quality of roosters with cashew-colored feather and roosters with banana-colored featherabout semen quality, rate of embryo, incubation rate. The objective of the thesis was to select rooster linesthat were suitable for Noi chicken hensin artificial insemination. The results showed that the sperm quality parameters of two chicken lines were relatively good. The semen color of two chicken lines is milky white. The proportion of eggs with embryos, hatching rate on hatching eggs were similar to two lines of rooster. The weight of one day-old chicks in roosters with cashew-colored feather was higher than others (P=0.001). From the results, it showed that two rooster lines were suitable for artificial insemination in farm conditions.