Gram-negative bacteria resistance to carbapenems conferred by New Delhi metallo-beta-Iactamase 1 (NDM-1) gene are a major global health problem. NDM-1 can be detected in bacteria isolated from both clinical samples as a river in Vietnam. In this study, the authors therefore assessed the prevalence of NDM-1 gene in a hospital environment. Various environmental ssamples (ie, hand nurse, medical trolley, floor surface patient bed or toilet cover site) were collected from three surgery departments (urology, gastro-enterology and hepato-biliary surgery) in a Hanoi hospital. Samples were transported to the microbiology laboratory and tested for carabapenem resistance and subsequently the presence of the NDM-l gene by PCR. NDM-l positive bacteria were further identified by biochemical testing (API-20E strip, Biomerieux) and susceptibility testing. The results showed that, in September 2011, 200 swab samples were collected from different enviroments. the authors detected 5/200 (2.5 percent) samples positive with the NDM-l gene: three samples were patient bed sheet, one was from cover of medical binwash disposal, and one in toilet cover. The positive samples were from Urology and Hepato-biliary Surgery departments, which also had NDM-l positive patients detected prior to this study. The NDM-l gene was not found in any samples from Gastro enterology department. Three species, including A. baumanii, Acinetobacter spp and E. aerogenes carrying NDM-l gene were found. All of positive NDM-l were extensively drug resistant, but remained susceptible to colistin.
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