Bacterial wilt disease caused by Rastonia solanacearum is a very serious disease which causes mass dead of crops in the field and this disease is very difficult to detect it in early stage and to control as well. Biological control is one of friendly enviromental methods in control disease. Some strains of Streptomyces were isolated and showed high antagonistic to bacterial wilt disease. One hundred fifty three strains of Streptomyces were isolated and selected from 16 soil samples collected from Thua Thien Hue, Quang Nam and Binh Dinh provinces. Number of actinomyces in the soil is range from 1.16 x 10 exponent 6 to 2.53 x 10 exponent 6 CFU/g soil. In which, number of Streptomyces strains have very high antagonistic to R. Solanacearum occupied 5,23 percent (Diameter of inhibition 20 mm) (Eight strains were selected). Three strains of Streptomyces were selected from 8 strains by direct selection method which showed the highest ability in inhibition of R. solanacearum were S3, S8, S26 with the diameter of inhibition zone 23.50; 26.83 and 24.66 mm, respectively. Species identification of these strains based on characteristics of morphology and 16S-rDNA sequences are S. parvulus, S. antibioticus, S. vinaceusdrappus.