The SWEET (sugars will be eventually exported transporters) family play important roles in various biological processes, such as plant growth, development and response to environmental conditions. In this study, we performed a comprehensive search of the cassava genome, leading to the identification of 28 members of MeSWEET gene family. These genes were distributed in 13 chromosomes out of 18 ones in cassava. Most of MeSWEET genes had 6 exons, this exon/intron organization was also previously found in other plant species. The isoelectric point and prediction using TargetP suggested that a majority of MeSWEET may be distributed on the secretory pathway. The results of this study would provide insights into the general information of SWEET gene family in cassava, thus serve as a basis for further functional research of such genes