This study aimed to examine differences in community composition, structure and diversity of dung beetles across various land uses in karst ecosystems of Huu Lien Nature Reserve. In total, 80 baited - pitfail traps were deployed and distributed across four main land uses of the study area: agricultural land (com fields), grasslands, Acacia plantations (10 years) and secondary forests (15 years) to collect dung beetles. In total, 36 dung - beetle species of 1647 individuals were recorded, of which, 27 species are new records for Lang Son Province. The examined land-uses showed significant differences in the species composition, abundance and Shannon diversity index of karst dung - beetles. Particularly, the community structure of dung beetles inhabiting the agricultural land statistically differed from those in the other land-uses, as the agricultural land dung beetles hosted diverse and abundant dung-beetle communities of small-bodied species (< 10 mm). For the first time, four indicator species of the agricultural land: Onthophagus {Gibbonthophagus) luridipennis Bohemann, 1858, Onthophagus (Parascatonomus) tricomis Wiedemann, 1823, Onthophagus (Gibbonthophagus) rectecomutus Lansberge, 1963 và Onthophagus (Phanaeomorphus) dorsofasciatus Fairmaire, 1893 and one unidentified indicator species of Acacia plantation: Onthophagus spl2 were identified.