Dioxin is one of the organic compounds that are difficult to decompose, causing environmental pollution and long-term effects on human health. The most reliable method for dioxin analysis is highresolution gas chromatography-mass spectrometry (HRGC/HRMS), but requires long analysis time, modern equipment, and high cost. Currently, immunoassay methods have been used to rapidly detect and screen for dioxin contamination in food and the environment. In particular, competitive ELISA is a method with high sensitivity, good signal amplification of low concentrations and can detect many different toxic congeners in the dioxin group. First, the antigen (hapten-BSA) was immobilized on a plate at 4°C overnight. After that, ELISA reaction was performed on the hapten-coated wells, at room temperature. The immobilization step of hapten-BSA onto the plate makes it difficult to analyze in the field, requires strict conditions and prolongs the analysis time. Therefore, the creation of coated plate with hapten-BSA needs to be investigated. In this study, coated plate with hapten-BSA was created with a hapten-BSA concentration of 0.5 µg/mL, using 5% sucrose as a preservative, BSA concentration of blocking buffer is 0.5% and with a TCDD detection threshold of 10 ppt in 12 month of storage. The successful creation of the coated plate with hapten-BSA shortens the analysis time to about 4-5 hours, which is convenient for analysis and get results in the field.

Dioxin is one of the organic compounds that are difficult to decompose, causing environmental pollution and long-term effects on human health. The most reliable method for dioxin analysis is highresolution gas chromatography-mass spectrometry (HRGC/HRMS), but requires long analysis time, modern equipment, and high cost. Currently, immunoassay methods have been used to rapidly detect and screen for dioxin contamination in food and the environment. In particular, competitive ELISA is a method with high sensitivity, good signal amplification of low concentrations and can detect many different toxic congeners in the dioxin group. First, the antigen (hapten-BSA) was immobilized on a plate at 4°C overnight. After that, ELISA reaction was performed on the hapten-coated wells, at room temperature. The immobilization step of hapten-BSA onto the plate makes it difficult to analyze in the field, requires strict conditions and prolongs the analysis time. Therefore, the creation of coated plate with hapten-BSA needs to be investigated. In this study, coated plate with hapten-BSA was created with a hapten-BSA concentration of 0.5 µg/mL, using 5% sucrose as a preservative, BSA concentration of blocking buffer is 0.5% and with a TCDD detection threshold of 10 ppt in 12 month of storage. The successful creation of the coated plate with hapten-BSA shortens the analysis time to about 4-5 hours, which is convenient for analysis and get results in the field.