The study was carried out to detennine the relationship between canxi and the incidence of inner gamboge disorder of mangosteen fruits, including two experiments: (1) investigated the relationship between calcium content in soil and flesh with inner gamboge disorder ratio on 30 different mangosteen orchards, age of trees from twenty to twenty-five years and (2) investigated the effect of calcium chloride foliar spray on inner gamboge disorder ratio, trees from twenty to twenty-five years were used in this experiment, spraying from 0.25 to 2 percent CaCl2 at bloom and 8 weeks after bloom, 4 applied times and at fifteen-day intervals. Results showed that: having a negative relation between the inner gamboge disorder ratio with exchangeable Ca2+ in soil and total Ca2+ in the flesh (r** = - 0.44 and - 0.52 respective), total Ca2+ content in flesh of normal fruits were higher in inner gamboge disorder fruits 1.29 fold. Spraying with 2 percent CaCl2 at 8 weeks after bloom hadn't effect but spraying at bloom reduced inner gamboge disorder and translucent flesh disorder ratio compared with no spraying due to increasing the total Ca2+ content in the flesh and pectin ratio in the cell wall, but also reduced fruit weight and yield per tree.