Nhân giống cây tam thất gừng (Stablianthus thorelli Gagnep) bằng phương pháp In Vitro

Chỉ số đề mục

68.35.03

Lĩnh vực nghiên cứu

Trồng trọt

Dạng tài liệu

Tác giả

Lã Văn Hiền⁽¹⁾, Dương Hữu Khoa, Bùi Đình Lãm, Ngô Xuân Bình

Nhan đề

Nhân giống cây tam thất gừng (Stablianthus thorelli Gagnep) bằng phương pháp In Vitro

Nhan đề tiếng anh

Propagation of plant notogingseng (Stablianthus thorelli Gagnep) by in-vitro method

Nguồn trích

Khoa học và công nghệ (Đại học Thái Nguyên

Năm xuất bản

2015

Số

12

Trang

37-44

ISSN

1859-2171

Từ khóa

Tam thất gừng, Nhân giống, In Vitro, Stablianthus thorelli

Từ khóa tiếng anh

Tóm tắt

Tóm tắt tiếng anh

Study on the regeneration capacity of notoginseng (Stablianthus thorelli Gagnep) was performed on MS (Murashige Skoog) medium that supplemented various growth regulations (BAP, kinetin, TDZ, and NAA). The results showed that the highest of sterilization efficiency of tubers (71.11 percent) was obtained at treatment of HgCl2 0.1 percent for 10min. The efficiency of shoot regeneration was the highest on MS medium with BAP 5.0mg/l of supplement, 108 shoots were generated after 14 days of culture. The effect of BAP, kinetin, TDZ, and NAA on shoot multiplication of notoginseng were determined: (i) supplement of BAP 5.0mg/l and NAA 0.5mg/l into MS medium could be generated 4.31 shoots/sample; (ii) the number of shoot regeneration was increased when adding of either kinetin 0.3mg/l or TDZ 0.5mg/l into MS medium containing BAP 5.0mg/l and NAA 0.5mg/l, 4.71 and 5,11 shoots/sample respectively. Plants were then transplanted in mixture soil (soil and rice husk, 3:1).

Kí hiệu kho

TTKHCNQG, CTv 178

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