Triglycerides present in wastewater are difficult to remove and degrade because they are difficult to dissolve in water. Clogging of wastewater pipes often occurs in triglyceride-containing wastewater treatment systems owing to the lipids present in the wastewater. For environmental conservation, microbial functions have been investigated with respect to their use in treating triglyceride-containing wastewater. Four bacterial strains using triglyceride as a carbon and energy sources were isolated from wastewater at Nam Son landfills, Soc Son, Hanoi. Amongst, the strain of BNST1 has shown as the best of growth strain in Gost medium supplemented with 1 percent of triglyceride. BNST1 strain has round colonies, milky color, smooth, convex, with the size from 1.5 to 2 mm in diameter. Under scanning electron microscopy with 15 thousand times, the cells have short rod with 0.5-0.8 um in wide and 1.0-1.5 um in length. Comparing of the part of 16S rRNA of the strain with bacterial strains in NCBI and LNSP by using universal primer pair 27f and 1527r, this strain belonged to Enlerobacler then was named Enterobacler sp. T1. The gene was registed in Genbank with accession number KF434761. The optimal conditions for the strain growth were at pH 7, 35°C and NaCI concentration of 0.5 percent after 7 incubated days. Using these optimal conditions, the T1 could grow in liquid medium supplemented with 12 percent triglyceride. The Enlerobacler sp. T1 degraded 63.2 percent of triglyceride after 7 days with the initial concentration of 13640 mg/l. This study suggests the potential usefulness of the Enterobacler sp. T1 to remove triglyceride from wastewater by bioremediation.
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